THRA antibody (N-Term)

Catalog No. ABIN6657352

Supplier Product No.: 209-301-e67

Product Details anti-THRA Antibody

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB)

Supplier Product No.: 209-301-e67

Supplier: Rockland

Purpose: Thyroid Hormone Receptor alpha 1/2 Antibody

Cross-Reactivity (Details): Anti-Thyroid Hormone Receptor alpha 1/2 antibody is directed against human Thyroid Hormone Receptor alpha 1/2 protein and is specific for the TR-α1 and the TR-α2 proteins.

Purification: Anti-Thyroid Hormone Receptor alpha 1/2 Antibody was purified by Protein G chromatography from cell culture supernatant followed by extensive dialysis against the buffer stated above.

Immunogen: Anti-Thyroid Hormone Receptor alpha 1/2 Monoclonal Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region of Thyroid Hormone Receptor alpha.

Clone: 2103

Isotype: IgG1

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1,000

Comment:

Thyroid Hormone Receptor alpha 1/2 antibody has been tested for use in Western Blot. The expected bands in western blot are 50kDa for TR-α1 and 58 kDa for the TR-α2 proteins. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA (THRA Products)

Background:

Synonyms: mouse anti-Thyroid Hormone Receptor alpha 1/2 Antibody, mouse anti-Thra antibody, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, EAR-7, c-erbA-1, c-erbA-alpha

Background: Thyroid Hormone Receptor alpha 1/2 antibody detects TRα1 and TRα2. Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory. Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRα1, TRα2, TRβ1 and TRβ2. TRα1, TRβ1 and TRβ2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain. Anti-TRα1/ TRα2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: THRA

Gene ID: 7067

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development