Goat anti-Rat (Rattus) IgG Antibody (DyLight 488) - Preadsorbed

Details for Product No. ABIN6699143
Antigen
Reactivity
Rat (Rattus)
355
342
337
232
232
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
336
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
DyLight 488
236
220
209
150
147
130
75
48
48
35
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Rat IgG, whole molecule

Isotype IgG
Characteristics

Synonyms: Goat Anti-Rat IgG DyLight 488™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 488™ Conjugation

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
Labeling Ratio 5.2
Antigen
Application Notes

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Rat (Rattus) IgG antibody (DyLight 488) - Preadsorbed (ABIN6699143) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody. Lane M: 3 μl Molecular Ladder. La...
Image no. 2 for Goat anti-Rat (Rattus) IgG antibody (DyLight 488) - Preadsorbed (ABIN6699143) Western Blot of 488 conjugated Goat Anti-Rat IgG secondary antibody. Lane 1: Rat IgG....