Goat anti-Rat IgG Antibody (DyLight 649) - Preadsorbed Secondary Antibody
- DyLight 649
- FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Synonyms: Goat Anti-Rat IgG DyLight 649™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649™ Conjugation
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
Immunogen: Rat IgG, whole molecule
- Labeling Ratio
- Application Notes
Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- For Research Use only
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
Riedemann, Straub, Sutor: "Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200567, 2019 (PubMed).
- Riedemann, Straub, Sutor: "Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200567, 2019 (PubMed).