Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Goat anti-Rat IgG Antibody (DyLight 649) - Preadsorbed Secondary Antibody

FLISA, FM, WB Polyclonal IgG DyLight 649
Pubmed (1)
Catalog No. ABIN6699155
$95.00
Plus shipping costs $45.00
100 μg ABIN6699155
100 μg ABIN6699155
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    IgG
    Reactivity
    Rat
    • 367
    • 335
    • 315
    • 223
    • 214
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Goat
    • 829
    • 781
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1795
    • 177
    Conjugate
    DyLight 649
    Application
    FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
    Characteristics

    Synonyms: Goat Anti-Rat IgG DyLight 649™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649™ Conjugation

    Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Purification
    Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
    Immunogen

    Immunogen: Rat IgG, whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.9
  • Application Notes

    Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Riedemann, Straub, Sutor: "Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200567, 2019 (PubMed).

  • Target
    IgG
You are here:
help Support