GST antibody (DyLight 649)

Catalog No. ABIN6720733

Product Details anti-GST Antibody (DyLight 649)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Tag

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is conjugated to DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST (Schistosoma japonicum) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Glutathione-S-Transferase (GST).

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 1.8

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: 1:10,000 - 1:25,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

References for anti-GST antibody (DyLight 649) (ABIN6720733)

Nascimbeni, Codogno, Morel: "Local detection of PtdIns3P at autophagosome biogenesis membrane platforms." in: Autophagy, Vol. 13, Issue 9, pp. 1602-1612, (2018) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Synonyms: GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody

Background:

Synonyms: goat anti-GST antibody DyLight™ 649 conjugation, DyLight™ 649 conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody

Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.