PSMA1 antibody (AA 170-219)

Catalog No. ABIN6736748

Product Details anti-PSMA1 Antibody

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Binding Specificity: AA 170-219

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Cow, Xenopus laevis, Pig, Chicken, Hamster, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PSMA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Horse, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa170-219 of human PSMA1 (P25786, NP_002777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Xenopus, Trout, Catfish, Salmon, Smelt, Stickleback, Pike, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PSMA1

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Alternative Name: PSMA1 (PSMA1 Products)

Background: Name/Gene ID: PSMA1
Subfamily: Threonine T1
Family: Protease Non Catalytic

Synonyms: PSMA1, 30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PSC2, PROS30, Proteasome nu chain, Proteasome subunit alpha 1, Proteasome subunit nu, Protein P30-33K, Macropain subunit nu, PROS-30, Proteasome component C2

Gene ID: 5682

NCBI Accession: NP_002777

UniProt: P25786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA