ASNA1 antibody (AA 218-267)

Catalog No. ABIN6746776

Product Details anti-ASNA1 Antibody

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Binding Specificity: AA 218-267

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASNA1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ASNA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human ASNA1 (O43681, NP_004308). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus, Stickleback, Zebrafish (100%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ASNA1 antibody (ABIN6746776)

Ordentlich, Downes, Xie, Genin, Spinner, Evans: "Unique forms of human and mouse nuclear receptor corepressor SMRT." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 2639-44, (1999) (PubMed).

Wagner, Norris, Knotts, Weigel, McDonnell: "The nuclear corepressors NCoR and SMRT are key regulators of both ligand- and 8-bromo-cyclic AMP-dependent transcriptional activity of the human progesterone receptor." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1369-78, (1998) (PubMed).

Nagy, Kao, Chakravarti, Lin, Hassig, Ayer, Schreiber, Evans: "Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase." in: Cell, Vol. 89, Issue 3, pp. 373-80, (1997) (PubMed).

Chen, Evans: "A transcriptional co-repressor that interacts with nuclear hormone receptors." in: Nature, Vol. 377, Issue 6548, pp. 454-7, (1995) (PubMed).

Target Details for ASNA1

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Alternative Name: ASNA1 (ASNA1 Products)

Background: Name/Gene ID: ASNA1
Subfamily: Arsenite resistance efflux pump
Family: Transporter

Synonyms: ASNA1, Arsenical pump-driving ATPase, ASNA-I, ARSA-I, ARSA1, Arsenite-stimulated ATPase, ATPase ASNA1, Golgi to ER traffic 3 homolog, HARSA-1, GET3, HASNA-1, HASNA-I, TRC40, Arsenite-translocating ATPase, HARSA-I

Gene ID: 439

NCBI Accession: NP_004308

UniProt: O43681

Pathways: Positive Regulation of Peptide Hormone Secretion