AIF1 antibody (Allograft Inflammatory Factor 1) (AA 116-147)

Details for Product anti-AIF1 Antibody No. ABIN685477
Antigen
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • Bart1
  • iba1
  • mrf-1
  • fa04b11
  • wu:fa04b11
  • AIF
  • AIF1
  • aif1
  • allograft inflammatory factor 1
  • AIF1
  • Aif1
  • aif1
Alternatives
anti-Human AIF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 116-147
34
25
16
14
14
9
8
7
6
6
4
4
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
228
113
108
9
9
2
2
1
1
Host
Rabbit
163
58
42
1
1
Clonality
Polyclonal
Conjugate
This AIF1 antibody is un-conjugated
32
25
11
5
5
5
5
5
5
4
4
4
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
191
129
125
43
19
15
12
11
10
6
4
3
2
1
1
Options
'Independent Validation' Badge
Lot Number 980993W
Method validated Immunofluorescence
Positive Control Mouse spleen
Negative Control Mouse duodenum
Notes Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.
Primary Antibody
  • Antibody: AIF1
  • Catalog number: ABIN685477
  • Lot number: 980993W
Isotype Control Antibody
  • Antibody: Rabbit IgG isotype control
  • Batch number: SLBD3695V
Secondary Antibody
  • Antibody: Alexa Fluor 546 Donkey Anti Rabbit
  • Lot number: 1218269
Controls
  • Positive control: Wild type mouse spleen (specimen known to contain the target protein) from Explora BioLabs.
  • Negative Control: Wild type mouse duodenum (specimen known to not contain the target protein) from Explora BioLabs.
  • Primary antibody isotype control: Wild type mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse spleen treated with secondary antibody only (no primary antibody).
Protocol
  • Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
  • Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
  • Fixed slides were rinsed three times in PBS for 5 min each at RT.
  • Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
  • Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
  • Sections were rinsed three times in PBS for 5 min each at RT.
  • Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
  • Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
  • Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.
Experimental Notes None
Validation Images
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (b...
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (bl...
Immunogen KLH conjugated synthetic peptide derived from human AIF1
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others AIF1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Aif1 (AIF1 Antibody Abstract)
Background

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Gene ID 199
UniProt P55008
Pathways Smooth Muscle Cell Migration
Application Notes WB 1:100-1000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclo...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclona...
Immunofluorescence (IF) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclon...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclon...
 image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) BV-microglia stained with AIF1 Polyclonal Antibody at 1:100 dilution. AIF1 expressio...
 image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal A...
Product cited in: Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)). Further details: Sample: spinal cord

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: brain

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, 2017 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Mouse (Murine)).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

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