Occludin antibody (OCLN) (AA 480-520)

Details for Product anti-OCLN Antibody No. ABIN687337, Supplier: Log in to see
Antigen
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • tpmt
  • OCLN
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • BLCPMG
  • occludin
  • occludin S homeolog
  • occludin a
  • Ocln
  • OCLN
  • ocln.S
  • ocln
  • oclna
Alternatives
anti-Rat (Rattus) Occludin antibody for Immunohistochemistry
Epitope
AA 480-520
30
14
12
6
6
6
5
4
4
4
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
125
70
60
17
15
4
4
4
3
2
2
2
Host
Rabbit
105
28
3
Clonality
Polyclonal
Conjugate
This Occludin antibody is un-conjugated
7
7
6
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
118
62
44
28
19
16
12
8
7
2
1
1
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Lot Number 990698W
Method validated Immunofluorescence
Positive Control Mouse stomach tissue
Negative Control Human fat tissue
Notes Signal was detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antibody: Occludin (OCLN)
  • Catalog number: ABIN687337
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 990698W
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: PPFE mouse stomach tissue
  • Negative Control: PPFE human fat tissue
  • Isotype antibody control: tissue sections treated with Rabbit IgG Isotype at 1ug/ul , Bioss bs-0295p
  • Secondary antibody only control: positive and negative control tissue sections treated with Goat anti-Rabbit Alexa 647 secondary antibody only.
Protocol
  • Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
  • The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
  • Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
  • Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
  • Tissue sections were rinsed three times in TBS for 5 min each at RT.
  • Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
  • IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
Experimental Notes
  • No challenges notes
  • OCLN is recommended to staining of mouse stomach tissue according to this protocol.
Validation Images
Immunofluorescence validation image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (...
Immunogen KLH conjugated synthetic peptide derived from mouse Occludin
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others Occludin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Occludin (OCLN Antibody Abstract)
Background

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subcellular location: Cytoplasm

Synonyms: Ocl, AI53564, Occludin, Ocln

Gene ID 18260
UniProt Q61146
Pathways Cell-Cell Junction Organization, Hepatitis C
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (IHC) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
Immunofluorescence (IF) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Western Blotting (WB) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin P...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (AA 480-520) antibody (ABIN687337) Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody...
Product cited in: Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Brain

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, 2014 (PubMed). (Sample species: Pig (Porcine)).

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, 2013 (PubMed). (Sample species: Pig (Porcine)).

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, 2012 (PubMed). Method employed by authors: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Pig (Porcine)). Further details: Sample: Mid-jejunum fragments

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, 2011 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: brain

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