Occludin antibody (AA 282-415)

Catalog No. ABIN7477543

Product Details anti-Occludin Antibody

Target: Occludin (OCLN)

Binding Specificity: AA 282-415

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Clone: MSVA-415M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin (OCLN Products)

Synonyms: AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody

Background: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C