CD74 antibody (HLA-DR-gamma)

Details for Product anti-CD74 Antibody No. ABIN6941256
Antigen
  • DHLAG
  • HLADG
  • II
  • Ia-GAMMA
  • CLIP
  • Ii
  • INVG34
  • Iclp-1
  • iclp1
  • wu:fb11f09
  • wu:fb12a01
  • wu:fb13d06
  • wu:fk94e07
  • CD74 molecule
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain a
  • CD74
  • Cd74
  • cd74a
Reactivity
Human
481
103
31
27
Host
Mouse
408
104
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is un-conjugated
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7
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5
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4
4
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4
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Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
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Options
Immunogen Recombinant full-length human CD74 protein
Clone CLIP-813
Isotype IgG1, kappa
Specificity It recognizes a protein of ~35 kDa, identified as CD74. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Purification Purified by Protein A/G
Plasmids, Primers & others Plasmids, Primers & others CD74 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Molecular Weight 33-41kDa
Gene ID 972
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Application Notes

Positive Control: Daudi or Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells) (Methanol Fixed), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions For Research Use only
Concentration 200 μg/mL
Buffer 10 mM PBS with 0.05 % BSA & 0.05 % azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-80 °C
Storage Comment Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Expiry Date 24 months
Supplier Images
Image no. 1 for anti-HLA-DR-gamma (CD74) antibody (ABIN6941256) Immunofluorescent staining of Raji cells. CD74 Mouse Monoclonal Antibody (CLIP/813) f...
Image no. 2 for anti-HLA-DR-gamma (CD74) antibody (ABIN6941256) Flow Cytometric Analysis of Human Raji cells. CD74 Mouse Monoclonal Antibody (CLIP/81...
Image no. 3 for anti-HLA-DR-gamma (CD74) antibody (ABIN6941256) Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Ant...
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