Protein O-GlcNAcase (MGEA5) antibody

Details for Product No. ABIN6944352
Target
Reactivity
Human, Mouse
2
2
Host
Rabbit
2
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
2
2
2
Options
Immunogen Recombinant protein of N-terminal human MGEA5.
Clone 1A5
Isotype IgG
Purification Purified by Protein A.
Target
Alternative Name OGA
Background

Isoform 1: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: - Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

Subcellular location: Cytoplasm, Nucleus


Synonyms: Protein O-GlcNAcase, Beta-N-acetylglucosaminidase, Beta-N-acetylhexosaminidase, Beta-hexosaminidase, Meningioma-expressed antigen 5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, OGA, NCOAT, MGEA5, HEXC, KIAA0679, MEA5
Gene ID 10724
UniProt O60502
Application Notes WB(1:500-2000), IHC-P(1:50-200), IP(1:10-50)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months