PAK1/2/3 antibody (pSer139, pSer141, pSer144)

Catalog No. ABIN6944410

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: pSer139, pSer141, pSer144

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: Synthetic phospho-peptide surrounding S139+S141+S144 of PAK

Clone: 9A3

Isotype: IgG

Application Details

Application Notes: WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200), IHC()

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1, PAK2, PAK3 (PAK1/2/3 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody

Background:

Synonyms: Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK, Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-1, PAK1, PAK-2, PAK2, PAK-3, PAK3

Background: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Gene ID: 5063

UniProt: O75914