POLR2A/RPB1 antibody (pSer2)
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- Target See all POLR2A/RPB1 (POLR2A) Antibodies
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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Binding Specificity
- pSer2
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This POLR2A/RPB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- Synthetic phospho-peptide corresponding to residues surrounding Ser2 of human POLR2A
- Clone
- 1C1
- Isotype
- IgG
- Top Product
- Discover our top product POLR2A Primary Antibody
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- Application Notes
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC() - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
- Alternative Name
- POLR2A (POLR2A Products)
- Background
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Synonyms: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, POLR2A
Background: RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
- Gene ID
- 5430
- UniProt
- P24928
- Pathways
- Regulatory RNA Pathways
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