APOB antibody (Biotin)

Catalog No. ABIN6963945

Product Details anti-APOB Antibody (Biotin)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOB antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Native apoB in the form of LDL.

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with apoB from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Human LDL

Clone: LDL 11

Isotype: IgG1

Application Details

Application Notes: For quantification of apoB in serum/plasma samples and cell culture supernatants using ELISA. LDL 11 is recommended as detection mAb in combination with coating mAb LDL 20/17 . The antibody is also suitable for immunoprecipitation and Western blot.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Expiry Date: 18 months

References for anti-APOB antibody (Biotin) (ABIN6963945)

Yu, Rimbert, Palmer, Toyohara, Xia, Xia, Ferreira, Chen, Chen, Loaiza, Horwitz, Kacergis, Zhao, Soukas, Kuivenhoven, Kathiresan, Cowan: "GPR146 Deficiency Protects against Hypercholesterolemia and Atherosclerosis." in: Cell, Vol. 179, Issue 6, pp. 1276-1288.e14, (2020) (PubMed).

Cayo, Mallanna, Di Furio, Jing, Tolliver, Bures, Urick, Noto, Pashos, Greseth, Czarnecki, Traktman, Yang, Morrisey, Grompe, Rader, Duncan: "A Drug Screen using Human iPSC-Derived Hepatocyte-like Cells Reveals Cardiac Glycosides as a Potential Treatment for Hypercholesterolemia." in: Cell stem cell, Vol. 20, Issue 4, pp. 478-489.e5, (2017) (PubMed).

Vu, Bemis, Benson, Bista, Carney, Fahrner, Lee, Liu, Lonkar, Milne, Nichols, Picarella, Shoelson, Smith, Ting, Wensley, Yeager, Zimmer, Jirousek: "Synthesis and Characterization of Fatty Acid Conjugates of Niacin and Salicylic Acid." in: Journal of medicinal chemistry, Vol. 59, Issue 3, pp. 1217-31, (2016) (PubMed).

Bissig-Choisat, Wang, Legras, Saha, Chen, Bell, Pankowicz, Hill, Barzi, Leyton, Leung, Kruse, Himes, Goss, Wilson, Chan, Lagor, Bissig: "Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model." in: Nature communications, Vol. 6, pp. 7339, (2015) (PubMed).

Derwall, Malhotra, Lai, Beppu, Aikawa, Seehra, Zapol, Bloch, Yu: "Inhibition of bone morphogenetic protein signaling reduces vascular calcification and atherosclerosis." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 32, Issue 3, pp. 613-22, (2012) (PubMed).

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: apoB (APOB Products)

Gene ID: 338

Pathways: Lipid Metabolism