APOB antibody (Apolipoprotein B)

Details for Product anti-APOB Antibody No. ABIN968961
Target
Reactivity
Human
144
32
25
8
4
1
1
Host
Mouse
73
55
34
4
4
Clonality (Clone)
Monoclonal ()
Conjugate
This APOB antibody is un-conjugated
24
18
10
8
7
6
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
86
81
39
34
18
17
14
13
12
10
9
9
4
4
2
2
1
1
1
1
1
1
Options
Immunogen Purified recombinant fragment of human ApoB expressed in E. Coli.
Clone 6G6
Isotype IgG1
Purification Ascites
Target
Alternative Name ApoB (APOB Antibody Abstract)
Background This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Molecular Weight 516 kDa
Gene ID 338
HGNC 338
Pathways Lipid Metabolism
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Images
Image no. 1 for anti-Apolipoprotein B (APOB) antibody (ABIN968961) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Image no. 2 for anti-Apolipoprotein B (APOB) antibody (ABIN968961) Figure 3: Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green) . B...
Image no. 3 for anti-Apolipoprotein B (APOB) antibody (ABIN968961) Figure 4: Western blot analysis using ApoB mAb against human ApoB (AA: 3900-4110) rec...
Image no. 4 for anti-Apolipoprotein B (APOB) antibody (ABIN968961) Figure 2: Flow cytometric analysis of serun using ApoB mouse mAb (green) and negative...
Product cited in: Benn: "Apolipoprotein B levels, APOB alleles, and risk of ischemic cardiovascular disease in the general population, a review." in: Atherosclerosis, Vol. 206, Issue 1, pp. 17-30, 2009 (PubMed).

Peterson, Mack, Hall, Alsup, Alexander, Sully, Sawires, Cheung, Otto, Gresham: "Apolipoprotein B Is an innate barrier against invasive Staphylococcus aureus infection." in: Cell host & microbe, Vol. 4, Issue 6, pp. 555-66, 2008 (PubMed).