RAPTOR antibody (AA 910-960)

Catalog No. ABIN6990531

Product Details anti-RAPTOR Antibody

Target: RAPTOR

Binding Specificity: AA 910-960

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Raptor has multiple isoforms that may also be recognized by antibody.

Purification: Raptor Antibody is affinity chromatography purified via peptide column.

Immunogen: Raptor antibody was raised against a 15 amino acid synthetic peptide from the central region of human Raptor. The immunogen is located within amino acids 910 - 960 of Raptor.

Isotype: IgG

Application Details

Application Notes: Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Raptor Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Raptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for RAPTOR

Target: RAPTOR

Alternative Name: Raptor (RAPTOR Products)

Background: Raptor Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif, mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Gene ID: 74370

UniProt: Q8K4Q0

Pathways: Warburg Effect