Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
At least four isoforms of EIG121 known to exist. EIG121 antibody is predicted to not cross-react with other UPF0577 protein family members.
Purification
EIG121 Antibody is affinity chromatography purified via peptide column.
Immunogen
EIG121 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human EIG121. The immunogen is located within the last 50 amino acids of EIG121.
EIG121 antibody can be used for detection of EIG121 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
EIG121 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
EIG121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
ELAPOR1
(Endosome/Lysosome-associated Apoptosis and Autophagy Regulator 1 (ELAPOR1))
EIG121 Antibody: EIG121 (Estrogen-induced gene 121 protein) is thought to play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma. It belongs to the UPF0577 family. It is a 1013 amino acid single-pass transmembrane protein that, though expressed in normal endometrium, is overexpressed in endometriod tumors. As an evolutionarily conserved gene, EIG121 is also expressed during early xenopus development, showing maximum expression at the gastrula stage.