VPS51 antibody (C-Term)

Catalog No. ABIN6991732

Product Details anti-VPS51 Antibody

Target: VPS51 (Vacuolar Protein Sorting 51 Homolog (VPS51))

Binding Specificity: AA 620-670, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS51 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: At least two alternatively spliced isoforms are known to exist, this antibody will detect both isoforms.

Purification: Fat Free Antibody is affinity chromatography purified via peptide column.

Immunogen: Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.

Isotype: IgG

Application Details

Application Notes: Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μ,g/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Fat Free Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Fat Free antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for VPS51

Target: VPS51 (Vacuolar Protein Sorting 51 Homolog (VPS51))

Alternative Name: Fat Free (VPS51 Products)

Background: Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

Molecular Weight: 86 kDa

Gene ID: 738

NCBI Accession: NP_037397

UniProt: Q9UID3