Rubicon antibody (N-Term)

Catalog No. ABIN6992053

Product Details anti-Rubicon Antibody

Target: Rubicon (KIAA0226) (KIAA0226)

Binding Specificity: AA 240-290, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubicon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Purification: Rubicon antibody is affinity chromatography purified via peptide column.

Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.

Isotype: IgG

Application Details

Application Notes: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rubicon antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Rubicon

Target: Rubicon (KIAA0226) (KIAA0226)

Alternative Name: Rubicon (KIAA0226 Products)

Background: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Molecular Weight: Predicted: 90, 101, 109 kDa

Observed: 91, 99 kDa

Gene ID: 9711

NCBI Accession: XP_005269431

UniProt: Q92622

Pathways: Autophagy