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beta Actin antibody (N-Term) (HRP)

ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Drosophila melanogaster WB, ELISA Host: Rabbit Polyclonal HRP

Catalog No. ABIN6992211

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
40

16

15

6

6

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
178

130

110

54

42

41

37

27

25

23

18

17

12

10

7

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat, Chicken, Rabbit, Drosophila melanogaster
Host
113

81

5

1

1
Rabbit
Compatible Secondaries
Clonality
112

87
Polyclonal
Conjugate
128

12

11

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is conjugated to HRP
Application
168

73

53

47

33

31

26

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), ELISA

Specificity

HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.

Purification

HRP-Beta-Actin antibody is affinity chromatography purified via peptide column.

Immunogen

HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

Isotype

IgG

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anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey WB, IF Host: Goat Polyclonal unconjugated

Application Notes

HRP-Beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ,/mL.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

HRP-Beta-Actin antibody is supplied in PBS containing 1 % BSA and 0.02 % thimerosol.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,4 °C

Storage Comment

HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

beta-Actin (ACTB Products)

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Molecular Weight

Predicted: 42 kDa

Observed: 42 kDa

Gene ID

60

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction