beta Actin antibody (N-Term)

Catalog No. ABIN6254200

This Goat Polyclonal antibody specifically detects beta Actin in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian and Donkey.

Quick Overview for beta Actin antibody (N-Term) (ABIN6254200)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Purpose: Anti-beta-Actin

Sequence: MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPE

Specificity: Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:2,000, IF:1:50-1:250, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-Actin

Background:

Actin proteins are highly conserved and ubiquitously expressed in all eukaryotic cells. These proteins are the major components of the cytoskeleton and involved in various types of cell motility.

ACTB, beta-actin, BRWS1, PS1TP5BP1 antibody.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction