POLD1 antibody (AA 41-254)

Catalog No. ABIN7444814

Product Details anti-POLD1 Antibody

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Binding Specificity: AA 41-254

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Polymerase DNA Directed Delta 1 (POLd)

Specificity: The antibody is a rabbit polyclonal antibody raised against POLd. It has been selected for its ability to recognize POLd in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Polymerase DNA Directed Delta 1 (POLd) corresdonding to Met41~Phe254 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:330-1300 Immunohistochemistry: 5-20 μg/mL

1:33-130 Immunocytochemistry: 5-20 μg/mL

1:33-130 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: Polymerase DNA Directed Delta 1 (POLD1 Products)

Background: CDC2, POLD1, DNA polymerase subunit delta p125

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA