HLA-DRB4 antibody

Catalog No. ABIN7246558

Product Details anti-HLA-DRB4 Antibody

Target: HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DRB4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human HLA-DRB4

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:20-1:100, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.02 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HLA-DRB4

Target: HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))

Alternative Name: HLA-DRB4 (HLA-DRB4 Products)

Synonyms: DR-4 antibody, DR4 antibody, DRB4 antibody, HLA-DR4B antibody, major histocompatibility complex, class II, DR beta 4 antibody, HLA-DRB4 antibody

Background: HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 30 kDa

UniProt: P13762

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Human Leukocyte Antigen (HLA) in Adaptive Immune Response