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beta Actin antibody

ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Monkey, Sheep, Xenopus laevis WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7250902
  • Target See all beta Actin (ACTB) Antibodies
    beta Actin (ACTB) (Actin, beta (ACTB))
    Reactivity
    • 177
    • 130
    • 110
    • 54
    • 42
    • 41
    • 37
    • 26
    • 25
    • 23
    • 17
    • 17
    • 12
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Rabbit, Monkey, Sheep, Xenopus laevis
    Host
    • 112
    • 81
    • 5
    • 1
    • 1
    Rabbit
    Clonality
    • 111
    • 87
    Polyclonal
    Conjugate
    • 126
    • 12
    • 12
    • 10
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This beta Actin antibody is un-conjugated
    Application
    • 167
    • 74
    • 52
    • 46
    • 32
    • 31
    • 26
    • 21
    • 13
    • 13
    • 8
    • 6
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Characteristics
    Control Antibody
    Purification
    Affinity purification
    Immunogen
    Recombinant Protein
    Isotype
    IgG
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    Discover our top product ACTB Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Avoid freeze / thaw cycles.
  • Target
    beta Actin (ACTB) (Actin, beta (ACTB))
    Alternative Name
    beta actin (ACTB Products)
    Background
    Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 
    Molecular Weight

    Observed_MW: 43 kDa

    Calculated_MW: 42 kDa

    UniProt
    P60709
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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