Adenosine A3 Receptor antibody (AA 216-230)

Catalog No. ABIN7042891

Product Details anti-Adenosine A3 Receptor Antibody

Target: Adenosine A3 Receptor (ADORA3)

Binding Specificity: AA 216-230

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A3 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Rat

Predicted Reactivity: Rat,mouse - 14,16 amino acid residues identical

Characteristics: Anti-Adenosine A3 Receptor Antibody is directed against an intracellular epitope of the human adenosine A3 receptor (A3AR). Anti-Adenosine A3 Receptor Antibody (ABIN7042891, ABIN7043892 and ABIN7043893)) can be used in western blot analysis, as well as immunocytochemical and immunohistocemical applications, and has been designed to recognize A3AR from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-AR004

Blocking Peptide: BLP-AR004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Adenosine A3 Receptor

Target: Adenosine A3 Receptor (ADORA3)

Alternative Name: ADORA3 (ADORA3 Products)

Background: ADORA3, A3AR, Adenosine A3R,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Alternative names: Adenosine A3 Receptor, ADORA3, A3AR, Adenosine A3R

Gene ID: 140

UniProt: P0DMS8

Pathways: Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process