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ADRA2A antibody (Extracellular)

ADRA2A Reactivity: Rat WB, IF, IHC, IF (cc) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7042899
  • Target See all ADRA2A Antibodies
    ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))
    Binding Specificity
    • 15
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 7-20, Extracellular
    Reactivity
    • 39
    • 34
    • 19
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 55
    • 3
    • 1
    Rabbit
    Clonality
    • 56
    • 3
    Polyclonal
    Conjugate
    • 24
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADRA2A antibody is un-conjugated
    Application
    • 35
    • 16
    • 13
    • 13
    • 11
    • 11
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))
    Purpose
    A Rabbit Polyclonal Antibody to α2A-Adrenergic Receptor
    Specificity
    Extracellular, N-terminus
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 13,14 amino acid residues identical
    Characteristics
    Anti-α2A-Adrenergic Receptor (extracellular) Antibody (ABIN7042899, ABIN7043918 and ABIN7043919)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2A-adrenoceptor from mouse, rat, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat alpha2A-adrenoceptor

    Isotype
    IgG
    Top Product
    Discover our top product ADRA2A Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:250

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IHC

    Negative Control: BLP-AR020

    Blocking Peptide: BLP-AR020

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))
    Alternative Name
    ADRA2A (ADRA2A Products)
    Background
    Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine, rat and mouse α2A displays less affinity compared to human3,4.

    Alternative names: alpha2A-Adrenergic Receptor, Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, alpha-2AAR, ADA2A, ADRA2A
    Gene ID
    25083
    NCBI Accession
    NM_000681
    UniProt
    P22909
    Pathways
    EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Negative Regulation of Transporter Activity
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