ADRA2A antibody (Extracellular)

Catalog No. ABIN7042899

This anti-ADRA2A antibody is a Rabbit Polyclonal antibody detecting ADRA2A in WB, IF, IHC and FACS. Suitable for Rat.

Quick Overview for ADRA2A antibody (Extracellular) (ABIN7042899)

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ADRA2A Antibody

Binding Specificity: AA 7-20, Extracellular

Purpose: A Rabbit Polyclonal Antibody to α2A-Adrenergic Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-α2A-Adrenergic Receptor (extracellular) Antibody (ABIN7042899, ABIN7043918 and ABIN7043919) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2A-adrenoceptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of rat alpha2A-adrenoceptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:250

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7234696)

Blocking Peptide: (ABIN7234696)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRA2A

Target: ADRA2A (Alpha-2A Adrenergic Receptor (ADRA2A))

Alternative Name: ADRA2A

Background: Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, α-2AAR, ADA2A, ADRA2A,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine, rat and mouse α2A displays less affinity compared to human3,4.

Alternative names: alpha2A-Adrenergic Receptor, Alpha-2A adrenoceptor, Alpha-2D adrenergic Receptor, alpha-2AAR, ADA2A, ADRA2A

Gene ID: 25083

NCBI Accession: NM_000681

UniProt: P22909

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Negative Regulation of Transporter Activity