Angiotensin II Type 2 Receptor antibody (Extracellular)

Catalog No. ABIN7042925

Product Details anti-Angiotensin II Type 2 Receptor Antibody

Target: Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))

Binding Specificity: AA 21-35, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiotensin II Type 2 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2

Specificity: Extracellular, N-terminal domain

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human AT2 receptor.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (ABIN7042925, ABIN7043902 and ABIN7043903)) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won't recognize human AT2R.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400-1:500

Comment:

Cited Application: IHC|ICC

Negative Control: BLP-AR012

Blocking Peptide: BLP-AR012

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Angiotensin II Type 2 Receptor

Target: Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))

Alternative Name: AGTR2 (AGTR2 Products)

Background: AT2 Receptor, AT2R, AGTR2, MRX88,Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.

Alternative names: Angiotensin II Receptor Type-2, AT2 Receptor, AT2R, AGTR2, MRX88

Gene ID: 24182

NCBI Accession: NM_000686

UniProt: P35351

Pathways: ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones