Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Angiotensin II Type 2 Receptor antibody (Extracellular) (Atto 488)

KO Validated AGTR2 Reactivity: Rat IHC, IF, LCI, IC Host: Rabbit Polyclonal Atto 488
Catalog No. ABIN7042926
  • Target See all Angiotensin II Type 2 Receptor (AGTR2) Antibodies
    Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
    Binding Specificity
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 21-35, Extracellular
    Reactivity
    • 48
    • 31
    • 30
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 43
    • 5
    • 1
    Rabbit
    Clonality
    • 35
    • 14
    Polyclonal
    Conjugate
    • 19
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Angiotensin II Type 2 Receptor antibody is conjugated to Atto 488
    Application
    • 30
    • 10
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)
    Purpose
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
    Specificity
    Extracellular, N-terminal domain
    Cross-Reactivity
    Mouse, Rat
    Cross-Reactivity (Details)
    Will not recognize human AT2 Receptor.
    Predicted Reactivity
    Mouse - identical
    Characteristics
    Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (ABIN7042925, ABIN7043902 and ABIN7043903)) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won't recognize human AT2R. \nAnti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#ABIN7042926) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging and immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.
    Purification
    Affinity purified on immobilized antigen.
    Grade
    KO Validated
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor

    Isotype
    IgG
    Top Product
    Discover our top product AGTR2 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20-1:50

    Application Dilutions Western blot wb: N/A

    Comment

    Negative Control: RIC-001-AG

    Blocking Peptide: BLP-AR012

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
    Alternative Name
    AGTR2 (AGTR2 Products)
    Background
    AT2 Receptor, AT2R, AGTR2, MRX88,Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.

    Alternative names: Angiotensin II Receptor Type-2, AT2 Receptor, AT2R, AGTR2, MRX88
    Gene ID
    24182
    NCBI Accession
    NM_000686
    UniProt
    P35351
    Pathways
    ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones
You are here:
Support