Angiotensin II Type 2 Receptor antibody (Extracellular) (Atto 488)
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- Target See all Angiotensin II Type 2 Receptor (AGTR2) Antibodies
- Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
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Binding Specificity
- AA 21-35, Extracellular
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Angiotensin II Type 2 Receptor antibody is conjugated to Atto 488
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)
- Purpose
- A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
- Specificity
- Extracellular, N-terminal domain
- Cross-Reactivity
- Mouse, Rat
- Cross-Reactivity (Details)
- Will not recognize human AT2 Receptor.
- Predicted Reactivity
- Mouse - identical
- Characteristics
- Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (ABIN7042925, ABIN7043902 and ABIN7043903)) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won't recognize human AT2R. \nAnti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#ABIN7042926) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging and immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.
- Purification
- Affinity purified on immobilized antigen.
- Grade
- KO Validated
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor
- Isotype
- IgG
- Top Product
- Discover our top product AGTR2 Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20-1:50
Application Dilutions Western blot wb: N/A
- Comment
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Negative Control: RIC-001-AG
Blocking Peptide: BLP-AR012
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
- Alternative Name
- AGTR2 (AGTR2 Products)
- Background
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AT2 Receptor, AT2R, AGTR2, MRX88,Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension. Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1. AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.
Alternative names: Angiotensin II Receptor Type-2, AT2 Receptor, AT2R, AGTR2, MRX88 - Gene ID
- 24182
- NCBI Accession
- NM_000686
- UniProt
- P35351
- Pathways
- ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones
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