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ATP2A1/SERCA1 antibody (Cytosolic)

ATP2A1 Reactivity: Rat WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7042953
  • Target See all ATP2A1/SERCA1 (ATP2A1) Antibodies
    ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))
    Binding Specificity
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 25-37, Cytosolic
    Reactivity
    • 41
    • 29
    • 20
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 50
    • 7
    Rabbit
    Clonality
    • 49
    • 8
    Polyclonal
    Conjugate
    • 31
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ATP2A1/SERCA1 antibody is un-conjugated
    Application
    • 43
    • 22
    • 16
    • 14
    • 14
    • 13
    • 13
    • 5
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
    Purpose
    A Rabbit Polyclonal Antibody to SERCA1 Pump
    Specificity
    Cytoplasmic, N-terminus
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 12,13 amino acid residues identical
    Characteristics
    Anti-SERCA1 Antibody (ABIN7042953 and ABIN7044150)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize SERCA1 from rat, mouse, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1

    Isotype
    IgG
    Top Product
    Discover our top product ATP2A1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: BLP-CP011

    Blocking Peptide: BLP-CP011

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))
    Alternative Name
    ATP2A1 (ATP2A1 Products)
    Background
    Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1,Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.The expression of SERCA1 in mice is essential3 whereas in human its absence is tolerated but is the cause of Brody myopathy4. Malfunction of SERCAs is also observed in heart disease and different forms of cancer1.

    Alternative names: SERCA1, Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1
    Gene ID
    116601
    NCBI Accession
    NM_004320
    UniProt
    Q64578
    Pathways
    Ribonucleoside Biosynthetic Process
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