ATP2B1 antibody (Intracellular)

Catalog No. ABIN7042955

Product Details anti-ATP2B1 Antibody

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Binding Specificity: AA 12-24, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to PMCA1 ATPase

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize all known PMCA1 splice variants.

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-PMCA1 Antibody is directed against an epitope of the human Plasma membrane calcium-transporting ATPase 1. Anti-PMCA1 Antibody (ABIN7042955, ABIN7044148 and ABIN7044149)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-CP005

Blocking Peptide: BLP-CP005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP2B1

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Alternative Name: ATP2B1 (ATP2B1 Products)

Background: Plasma membrane Ca2+ ATPase 1, ATP2B1,The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1.PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2.Since it transports Ca2+ into the extracellular space, PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3.The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4.The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, and so is suited to maintaining Ca2+ at its normally very low levels. The NCX is better suited to removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.

Alternative names: PMCA1, Plasma membrane Ca2+ ATPase 1, ATP2B1

Gene ID: 490

NCBI Accession: NM_001682

UniProt: P20020

Pathways: Ribonucleoside Biosynthetic Process