ATP2B1 antibody (AA 312-327)

Catalog No. ABIN870665

The Goat Polyclonal anti-ATP2B1 antibody is suitable to detect ATP2B1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for ATP2B1 antibody (AA 312-327) (ABIN870665)

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP2B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ATP2B1 Antibody

Binding Specificity: AA 312-327

Purpose: ATP2B1 (aa312-327)

Sequence: KQDGAIENRN KAKAQD

Specificity: This antibody is expected to recognize both reported isoforms (NP_001001323.1, NP_001673.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KQDGAIENRNKAKAQD, from the internal region of the protein sequence according to NP_001001323.1, NP_001673.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in lysates of cell line HeLa (calculated MW of 135 kDa according to NP_001673.2). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATP2B1

Target: ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))

Alternative Name: ATP2B1

Background: ATP2B1, ATPase, Ca++ transporting, plasma membrane 1, PMCA1, PMCA1kb, plasma membrane calcium ATPase, plasma membrane calcium pump, plasma membrane calcium-ATPase, plasma membrane calcium-transporting ATPase 1

Molecular Weight: 135kDa

Gene ID: 490, 67972, 29598

NCBI Accession: NP_001001323, NP_001673

Pathways: Ribonucleoside Biosynthetic Process