ATP2A3 antibody (Cytosolic)

Catalog No. ABIN7042959

Product Details anti-ATP2A3 Antibody

Target: ATP2A3 (ATPase, Ca++ Transporting, Ubiquitous (ATP2A3))

Binding Specificity: AA 981-993, Cytosolic

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to SERCA3 Pump

Specificity: Cytoplasmic, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - 11, human - 10,13 amino acid residues identical

Characteristics: Anti-SERCA3 Antibody (ABIN7042959, ABIN7044153 and ABIN7044154)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SERCA3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CP014

Blocking Peptide: BLP-CP014

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP2A3

Target: ATP2A3 (ATPase, Ca++ Transporting, Ubiquitous (ATP2A3))

Alternative Name: ATP2A3 (ATP2A3 Products)

Background: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3,SERCA3 belongs to the family of P-type ion translocating ATPases and is responsible for maintaining a Ca2+ concentration gradient between the cytosol and the endoplasmic reticulum. SERCA3 transports two Ca2+ ions in the endoplasmic reticulum (ER) per ATP molecule hydrolyzed1-3. SERCA proteins are transmembrane and consist of a single polypeptide chain folded into four major domains. SERCA proteins are encoded by 3 genes, ATP2A1, ATP2A2, and ATP2A3. All three genes produce several alternate transcripts and protein isoforms.SERCA3 protein is mostly expressed in non-muscle cells, especially in endothelial and hematopoietic cells. Minor expression can be detected in muscle cells2,3.SERCA3 knockout mice exhibit prolonged tail bleeding time and rebleeding1. Mutations in the SERCA3 gene are associated with type 2 diabetes. The protein is also implicated in several cardiac disorders2,3.

Alternative names: SERCA3, Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3

Gene ID: 25391

NCBI Accession: NM_174953

UniProt: P18596

Pathways: Myometrial Relaxation and Contraction, Ribonucleoside Biosynthetic Process