ATP2A3 antibody (N-Term)

Catalog No. ABIN3043794

The Rabbit Polyclonal anti-ATP2A3 antibody (ABIN3043794) specifically detects ATP2A3 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ATP2A3 antibody (N-Term) (ABIN3043794)

Target: ATP2A3 (ATPase, Ca++ Transporting, Ubiquitous (ATP2A3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATP2A3 Antibody

Binding Specificity: AA 1-30, N-Term

Purpose: Anti-SERCA3 ATPase/ATP2A3 Antibody

Sequence: MEAAHLLPAA DVLRHFSVTA EGGLSPAQVT

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SERCA3 ATPase/ATP2A3 Antibody (ABIN3043794). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP2A3, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATP2A3

Target: ATP2A3 (ATPase, Ca++ Transporting, Ubiquitous (ATP2A3))

Alternative Name: ATP2A3

Background:

Synonyms: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3,SERCA3,SR Ca (2+)-ATPase 3,3.6.3.8,Calcium pump 3,ATP2A3,

Tissue Specificity: Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Expressed in cell lineages of hematopoietic, epithelial, or embryonic origin and also expressed in several cancer cell lines. .

Background: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, also known as SERCA3, is anenzyme that in humans is encoded by the ATP2A3 gene. It is mapped to 17p13.2. This gene encodes one of the SERCA Ca2+-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of cells. ATP2A3 expression was originally described as non-muscular, but was recently observed in cardiomyocyte. What's more, the expression of ATP2A3 was significantly reduced or lost in colon carcinomas compared with normal colonic epithelial cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction.

Sequence Similarities: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Molecular Weight: 114 kDa

Gene ID: 489

UniProt: Q93084

Pathways: Myometrial Relaxation and Contraction, Ribonucleoside Biosynthetic Process