CALCRL antibody (Extracellular)

Catalog No. ABIN7042979

Product Details anti-CALCRL Antibody

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Binding Specificity: AA 127-140, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALCRL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Calcitonin Receptor-Like Receptor

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12,14 amino acid residues identical

Characteristics: Anti-CRLR/CALCRL (extracellular) Antibody (ABIN7042979, ABIN7044198 and ABIN7044199)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. It has been designed to recognize CRLR from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence:  (C)EQMQKVRGFR(S)GKKS, corresponding to amino acid residues 522 - 536 of mouse OCTN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR060

Blocking Peptide: BLP-CR060

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Alternative Name: CALCRL (CALCRL Products)

Background: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR,Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Alternative names: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Gene ID: 10203

NCBI Accession: NM_005795

UniProt: Q16602

Pathways: cAMP Metabolic Process