CACNB3 antibody (Intracellular)

Catalog No. ABIN7042987

Product Details anti-CACNB3 Antibody

Target: CACNB3 (Calcium Channel, Voltage-Dependent, beta 3 Subunit (CACNB3))

Binding Specificity: AA 463-477, Intracellular

Reactivity: Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to CACNB3 (CaV?3) Subunit

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Identical for all splice forms.

Predicted Reactivity: Rat,mouse,human,Xenopus - identical, bovine - 14,15 amino acid residues identical

Characteristics: Anti-CACNB3 Antibody is directed against an epitope of rabbit CaVβ3. Anti-CACNB3 Antibody (ABIN7042987, ABIN7043959 and ABIN7043960)) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody has been designed to recognize CaVβ3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-CC008

Blocking Peptide: BLP-CC008

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNB3

Target: CACNB3 (Calcium Channel, Voltage-Dependent, beta 3 Subunit (CACNB3))

Alternative Name: CACNB3 (CACNB3 Products)

Background: CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3,The β3 protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1-3 The structure of CaVβ3 was recently determined (as well as other CaVβ subunits, for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.1,2 CaVβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1

Alternative names: CACNB3, CaVbeta3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3

Gene ID: 100009402

NCBI Accession: NM_000725

UniProt: P54286

Pathways: Myometrial Relaxation and Contraction