CACNA1A antibody (Intracellular)

Catalog No. ABIN7043006

Product Details anti-CACNA1A Antibody

Target: CACNA1A (Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A))

Binding Specificity: AA 865-881, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel

Specificity: Intracellular loop between domains II and III

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 16,17 amino acid residues identical, human - 8

Characteristics: Anti-CACNA1A (CaV2.1) Antibody (ABIN7043006, ABIN7043949 and ABIN7043950) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CACNA1A 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|LCI

Negative Control: (ABIN7234985)

Blocking Peptide: (ABIN7234985)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNA1A

Target: CACNA1A (Calcium Channel, Voltage-Dependent, P/Q Type, alpha 1A Subunit (CACNA1A))

Alternative Name: CACNA1A (CACNA1A Products)

Background: Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI,Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent calcium channels are composed of several subunits, α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.1 (formally named α1A) makes up the α1 poreforming subunit in P/Q-type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with CaV2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,2Mutations in CACNA1A (CaV2.1) have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6).1,3-5The involvement of CaV2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA (#STA-500), a specific blocker of the CaV2.1 channel.6 The blocking sensitivity is dependent on the α subunit isoform and on the splice variant.7,8

Alternative names: CACNA1A (CaV2.1), Voltage-dependent P/Q-type calcium channel subunit alpha1A, Brain calcium channel I, BI

Gene ID: 25398

NCBI Accession: NM_000068

UniProt: P54282

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process