CXCR1 antibody (Extracellular)

Catalog No. ABIN7043037

Product Details anti-CXCR1 Antibody

Target: CXCR1 (Chemokine (C-X-C Motif) Receptor 1 (CXCR1))

Binding Specificity: AA 2-16, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor

Specificity: Extracellular, N-terminus

Predicted Reactivity: Specific to human CXCR1

Characteristics: Anti-Human CXCR1 (extracellular) Antibody is directed against an extracellular epitope of the human CXCR1. Anti-Human CXCR1 (extracellular) Antibody (ABIN7043037 and ABIN7044163)) can be used for western blot and indirect flow cytometry applications. It has been designed to recognize human CXCR1.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SNITDPQMW DFDDLN, corresponding to amino acid residues 2-16 of human CXCR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR011

Blocking Peptide: BLP-CR011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CXCR1

Target: CXCR1 (Chemokine (C-X-C Motif) Receptor 1 (CXCR1))

Alternative Name: CXCR1 (CXCR1 Products)

Background: C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181,Chemokine receptors belong to the seven transmembrane domain G-protein coupled receptor superfamily and are differentially expressed in diverse cell types. In leukocytes, chemokines coordinate development, differentiation, anatomic distribution, trafficking, and effector functions and thereby regulate innate and adaptive immune responses.To date, 18 chemokine receptors are known and designated as CXCR1-5, CCR1-11, XCR1, and CX3CR1 based on their specific chemokine preferences.The CXC chemokine receptors specifically bind and respond to cytokines of the CXC chemokine family. CXCR1 and CXCR2 were the first chemokine receptor subtypes to be defined. They are the only known mammalian receptors for ELR+ CXC chemokines [CXC chemokines which contain the tripeptide motif glutamic acid-leucine-arginine (ELR) N-terminal to the first cysteine].CXCR1 and CXCR2 both bind IL-8, with high affinity, they do not bind other types of chemokines. CXCR1 and CXCR2 are the major chemokine receptors expressed on neutrophils. They appear to operate mainly in acute inflammation and innate immunity.

Alternative names: CXCR1, C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181

Gene ID: 3577

NCBI Accession: NM_000634

UniProt: P25024

Pathways: cAMP Metabolic Process