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CLCN3 antibody (Intracellular)

KO Validated CLCN3 Reactivity: Rat WB, IHC, IF, IC, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043052
  • Target See all CLCN3 Antibodies
    CLCN3 (Chloride Channel 3 (CLCN3))
    Binding Specificity
    • 15
    • 11
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 592-661, Intracellular
    Reactivity
    • 33
    • 21
    • 15
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 31
    • 16
    Rabbit
    Clonality
    • 31
    • 16
    Polyclonal
    Conjugate
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CLCN3 antibody is un-conjugated
    Application
    • 46
    • 19
    • 13
    • 13
    • 13
    • 11
    • 10
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)
    Purpose
    A Rabbit Polyclonal Antibody to CLC-3 (CLCN3) Channel
    Specificity
    Intracellular, near the C-terminus
    Cross-Reactivity
    Human, Rat
    Predicted Reactivity
    rat CLC-5 - 49,70 amino acid residues identicalRat CLC-4 - 46, human,70 amino acid residues identical,rabbit,guinea pig - 69,Mouse - identical, Xenopus laevis - 61
    Characteristics
    Anti- (CLCN3) Antibody (ABIN7043052, ABIN7044121 and ABIN7044122) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from rat, mouse, and human samples.
    Purification
    The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
    Grade
    KO Validated
    Immunogen

    Immunogen: GST fusion protein

    Immunogen Sequence: GST fusion protein with the sequence SLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPR, corresponding to amino acid residues 592-661 of rat CLC-3

    Isotype
    IgG
    Top Product
    Discover our top product CLCN3 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IHC|ICC

    Negative Control: (ABIN7235086)

    Blocking Peptide: (ABIN7235086)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    0.2 mL double distilled water (DDW).
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    CLCN3 (Chloride Channel 3 (CLCN3))
    Alternative Name
    CLCN3 (CLCN3 Products)
    Background
    Chloride channel 3, Chloride transporter ClC-3, H+/Cl- exchange transporter 3,CLC-3 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-3 is widely distributed with prominent expression in tissues of neuroectoderm origin. In the brain, it is highly expressed in the hippocampus, olfactory bulb and olfactory cortex. The channel is also prominently expressed in aortic and coronary vascular smooth muscle cells, aortic endothelial cells and tracheal and alveolar epithelial cells.The physiological function of CLC-3 is not entirely clear, but it has been suggested that CLC-3 generates a shunt current of chloride for v-H+-ATPases, thereby aiding the acidification of endosomes and synaptic vesicles as well as lysosomes. Disruption of the ClC-3 gene in mice causes severe neuronal loss, leading to a complete loss of the hippocampus in adult mice. In addition, CLC-3 has been shown to have a critical role in the respiratory burst and phagocytosis of polymorphonuclear cells, a key cell type of innate host defense. 3,4

    Alternative names: CLC-3 (CLCN3), Chloride channel 3, Chloride transporter ClC-3, H+/Cl- exchange transporter 3
    Gene ID
    84360
    NCBI Accession
    NM_001243372
    UniProt
    P51792
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