CLCN3 antibody (C-Term)

Catalog No. ABIN2776327

There is 1 publication for this product available. The Rabbit Polyclonal anti-CLCN3 antibody is suitable to detect CLCN3 in samples from Human, Rat, Mouse, Guinea Pig, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio) and Sheep. It has been validated for WB and IHC.

Quick Overview for CLCN3 antibody (C-Term) (ABIN2776327)

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Reactivity: Human, Rat, Mouse, Guinea Pig, Rabbit, Dog, Horse, Cow, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLCN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CLCN3 Antibody

Binding Specificity: C-Term

Purpose: CLCN3 Antibody - C-terminal region

Sequence: VGDAFGREGI YEAHIRLNGY PFLDAKEEFT HTTLAADVMR PRRNDPPLAV L

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 91%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CLCN3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human CLCN3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8096909

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CLCN3 antibody (ABIN2776327)

Jensen, Nosjean, Dziegiel, Boutin, Sørensen, Karsdal, Henriksen: "A quantitative assay for lysosomal acidification rates in human osteoclasts." in: Assay and drug development technologies, Vol. 9, Issue 2, pp. 157-64, (2011) (PubMed).

Target Details for CLCN3

Target: CLCN3 (Chloride Channel 3 (CLCN3))

Alternative Name: CLCN3

Background:

Background Information: CLCN3 plays an important role in the cell proliferation of vascular smooth muscle cells. The PDZ-binding isoform of CLCN3 resides in the Golgi where it co-localizes with a small amount of CFTR (cystic fibrosis transmembrane conductance regulator).

Gene Name: Chloride channel, voltage-sensitive 3

Alternative Symbols: CLC3, ClC-3

Protein Name: H(+)/Cl(-) exchange transporter 3

Molecular Weight: 95kDa

Gene ID: 1182

NCBI Accession: NP_776297

UniProt: P51790