CRHR2 antibody (Extracellular)

Catalog No. ABIN7043088

Product Details anti-CRHR2 Antibody

Target: CRHR2 (Corticotropin Releasing Hormone Receptor 2 (CRHR2))

Binding Specificity: AA 20-34, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRHR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-CRF2/CRHR2 (extracellular) Antibody (ABIN7043088, ABIN7044188 and ABIN7044189)) is a highly specific antibody directed against an epitope of the rat Corticotropin-releasing factor receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CRHR2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EELLLDGWGEPPDPE, corresponding to amino acid residues 20-34 of rat CRHR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR052

Blocking Peptide: BLP-CR052

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CRHR2

Target: CRHR2 (Corticotropin Releasing Hormone Receptor 2 (CRHR2))

Alternative Name: CRHR2 (CRHR2 Products)

Background: Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B,CRFR2 is a G-protein coupled receptor for CRF (corticotropin-releasing factor) and urocortins UCN1, UCN2, and UCN3. The receptor-ligand interaction causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase, leading to increased intracellular cAMP levels1.CRFR2 is expressed in the CNS and peripheral tissues including the heart, vasculature, skeletal muscle, and gastrointestinal tract, and it plays a central role in coordinating endocrine, autonomic, and behavioral responses to stress1. CRFR2 ligands, the UCN peptides, exert actions in central and peripheral tissues where they modulate cardiovascular, immune, gastrointestinal, and reproductive functions in response to stress2. UCNs can also affect tumor biology as a consequence of the CRFR2 role as a suppressor of vascularization3.The involvement of CRF/UCN family peptides and their receptors in diverse pathophysiological states has generated intense interest in developing therapeutic agents that selectively target the receptors. CRFR2-selective agonists have the potential to treat heart failure4, hypertension5 and other disorders.CRFR2 shares 68 % amino acid sequence identity with another CRF receptor, CRFR1. Both of the receptors have a bipartite domain structure consisting of an N-terminal extracellular domain (ECD) of about 100-150 amino acids linked to a 7-transmembrane helical bundle domain embedded in the plasma membrane. Peptide binding spans the two domains, with the C-terminal portion of the peptide binding to the ECD to impart high affinity and the N-terminal portion binding to the 7-transmembrane domain to activate the receptor6.CRFR2 has three functional full-length isoforms, α, β, and γ, that differ only at the extreme N-terminus of the receptor. In humans, the CRFR2β isoform is expressed in the brain, and the CRFR2α isoform predominates in the periphery6.

Alternative names: Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B

Gene ID: 64680

NCBI Accession: NM_001202475

UniProt: P47866

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Feeding Behaviour