EPH Receptor A6 antibody (Extracellular)

Catalog No. ABIN7043125

This Rabbit Polyclonal antibody specifically detects EPH Receptor A6 in WB, IHC, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for EPH Receptor A6 antibody (Extracellular) (ABIN7043125)

Target: EPH Receptor A6 (Epha6)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-EPH Receptor A6 Antibody

Binding Specificity: AA 482-495, Extracellular

Purpose: A Rabbit Polyclonal Antibody to EphA6 Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-EphA6 (extracellular) Antibody (ABIN7043125, ABIN7044247 and ABIN7044248) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize EphA6 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7235290)

Blocking Peptide: (ABIN7235290)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for EPH Receptor A6

Target: EPH Receptor A6 (Epha6)

Alternative Name: EPHA6

Background: Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2,EphA6 is an erythropoietin-producing hepatocellular (Eph) receptor that belongs to the tyrosine kinase receptor family. The Eph receptor family consists of 9 EphA receptors and 5 EphB receptors, which serve key roles in normal physiology, including cell migration, cell adhesion, cell proliferation and neuronal synaptic plasticity. These activities depend on the binding of their Eph receptor-interacting (ephrin) proteins, of which include 8 members. Compelling data has demonstrated that Eph receptors and ephrins affect tumor growth, invasiveness, angiogenesis and metastasis1. They are also involved in brain development, learning and memory2.EphA6 signaling pathways are inhibitory for developing axons and blocking these pathways enhances regeneration following spinal cord and brain injury, rendering EphA6 an appealing drug target3.Eph receptors contain an extracellular structure consisting of an ephrin binding domain, the juxtamembrane region connects the extracellular portion of the receptor to the intracellular kinase domain. Two Tyrosine residues on the juxtamembrane region mediate autophosphorylation and receptor dimerization mediates the formation of heterocomplexes that are required for signaling4.

Alternative names: Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2

Gene ID: 29202

NCBI Accession: NM_001080448

UniProt: P54758

Pathways: RTK Signaling