GJB2 antibody (Intracellular)

Catalog No. ABIN7043203

Product Details anti-GJB2 Antibody

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Binding Specificity: AA 100-112, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Connexin-26

Specificity: Intracellular, cytoplasmic loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-Connexin-26 (GJB2) Antibody (ABIN7043203, ABIN7044090 and ABIN7044091)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Cx26 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC212

Blocking Peptide: BLP-CC212

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2 (GJB2 Products)

Background: CX26, Gap junction β2 protein,Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. Mutations in the gene encoding for Connexin-26 protein are associated with deafness7.

Alternative names: Connexin-26 (GJB2), CX26, Gap junction beta2 protein

Gene ID: 394266

NCBI Accession: NM_004004

UniProt: P21994

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization