GJD2 antibody (AA 170-182)

Catalog No. ABIN7043205

This anti-GJD2 antibody is a Rabbit Polyclonal antibody detecting GJD2 in WB, IHC and IF. Suitable for Rat.

Quick Overview for GJD2 antibody (AA 170-182) (ABIN7043205)

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GJD2 Antibody

Binding Specificity: AA 170-182

Purpose: A Rabbit Polyclonal Antibody to Connexin-36

Specificity: Intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-Connexin-36 Antibody (ABIN7043205, ABIN7044087 and ABIN7044088) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235554)

Blocking Peptide: (ABIN7235554)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJD2

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Alternative Name: GJD2

Background: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9,The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1-E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

Alternative names: Connexin-36, CX36, Gap junction delta2 protein, Gap junction alpha9 protein, GJD2, GJA9

Gene ID: 50564

NCBI Accession: NM_020660

UniProt: O70610