GJD2 antibody (Intracellular) (Atto 488)

Catalog No. ABIN7043206

Product Details anti-GJD2 Antibody (Atto 488)

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Binding Specificity: AA 170-182, Intracellular

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJD2 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-Connexin-36 Antibody (ABIN7043205, ABIN7044087 and ABIN7044088)) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples. \nAnti-Connexin-36-ATTO Fluor-488 Antibody (#ABIN7043206) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Connexin-36-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJD2

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Alternative Name: Connexin-36 (GJD2 Products)

Background: Alternative names: Connexin-36, CX36, Gap junction delta2 protein, Gap junction alpha9 protein, GJD2, GJA9

Gene ID: 50564

NCBI Accession: NM_020660

UniProt: O70610