GFRA3 antibody (Extracellular)

Catalog No. ABIN7043212

Product Details anti-GFRA3 Antibody

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Binding Specificity: AA 172-185, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to GDNF Family Receptor α3

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse,rat,human - 13,14 amino acid residues identical

Characteristics: Anti-GFR alpha 3 (extracellular) Antibody (ABIN7043212, ABIN7044779 and ABIN7044780)) is a highly specific antibody directed against an epitope of mouse GDNF receptor α3. The antibody can be used in western blot analysis. It has been designed to recognize GFR α3 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDRLRKAYGEA(S)SG, corresponding to amino acid residues 172-185 of mouse GFRA3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT023

Blocking Peptide: BLP-NT023

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GFRA3

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Alternative Name: GFRA3 (GFRA3 Products)

Background: GFRA3, GFRα3, GDNF family receptor α3, GDNF receptor alpha 3,GFRα3 (GDNF Family Receptor α3) belongs to the glial cell line derived neurotrophic factor (GDNF) family of receptors which contains four members GFRα1-4. The GDNF family of ligands (GFLs)- GDNF, Neurturin, Artemin, and Persephin bind to co-receptors GFRα1-4 and activate Ret receptor tyrosine kinase. GDNF-family receptor α is glycosyl-phosphatidylinositol anchored to the cell surface. GFRα3 structure contains three homologous domains (D1, D2 and D3) and a C-terminal extension1-4.GFRα3 is a highly selective receptor for Artemin (Artn). Artemin is a vasculature-derived neurotrophic factor that modulates the development and function of sensory neurons and participates in the pathophysiology of peripheral inflammation and pain hyperalgesia.In adults, GFRα3 is restricted to neurons of the peripheral nervous system. The protein is expressed by a subpopulation of nociceptive sensory neurons which also express the Ret receptor tyrosine kinase, TRPV1, TRPA11,2,3.

Alternative names: GFR alpha 3, GFRA3, GFRalpha3, GDNF family receptor alpha3, GDNF receptor alpha 3

Gene ID: 14587

NCBI Accession: NM_001496

UniProt: O35118