GFRA4 antibody (Extracellular, N-Term)

Catalog No. ABIN7043213

Product Details anti-GFRA4 Antibody

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Binding Specificity: AA 160-172, Extracellular, N-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Live Cell Imaging (LCI)

Cross-Reactivity (Details): Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.

Characteristics: Anti-GFR alpha 4 (extracellular) Antibody is directed against an extracellular epitope of rat GDNF receptor α4. Anti-GFR alpha 4 (extracellular) Antibody (ABIN7043213, ABIN7044781 and ABIN7044782)) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α4 from mouse and rat samples. The antibody is not suitable for detecting GFR α4 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GFRA4

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Alternative Name: GFR alpha 4 (GFRA4 Products)

Background: Alternative names: GFR alpha 4, GFRA4, GFRalpha4, GDNF family receptor alpha4, GDNF receptor alpha 4, Persephin receptor

Gene ID: 66023

NCBI Accession: NM_145762

UniProt: Q9EPI2