GLRA1 antibody (AA 350-362)

Catalog No. ABIN7043257

Product Details anti-GLRA1 Antibody

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Binding Specificity: AA 350-362

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Glycine Receptor α1

Specificity: 2nd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-Glycine Receptor alpha1 Antibody is directed against an epitope of the rat glycine receptor α1. Anti-Glycine Receptor α1 Antibody (ABIN7043257, ABIN7044394 and ABIN7044395) can be used in western blot and immunohistochemical staining. It has been designed to recognize GlyRα1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyRalpha1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235712)

Blocking Peptide: (ABIN7235712)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Alternative Name: GLRA1 (GLRA1 Products)

Background: Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1, Glyr,Glycine receptors (GlyRs) mediate ionotropic inhibitory neurotransmission in the CNS where they play an essential role in inhibition of motor neurons in the spinal cord and brainstem1. The glycine receptor is composed of three ligand-binding subunits of 48 kDa (α) and two homologous polypeptides of 58 kDa (β) that span the postsynaptic membrane in a pentameric arrangement to form a Cl--selective channel2. So far, at least three different α-subunit variants of the GlyR have been identified: α1, α2 and α33. Homo-oligomeric α2 receptors have a low strychnine-binding affinity and are considered to represent a neonatal form of GlyR, which is replaced during development by receptors containing the α1 subunit. Adult GlyRs are predominantly composed of α1 and β subunits, the α3 subunit being expressed at low levels in only a few brain regions4.GlyRα1 subunits are widely expressed and contribute to many processes in the CNS, including inflammatory pain perception, modulation of auditory and visual pathways, and neurotransmission in the cerebellar cortex5.Mutations in glycine receptor α1 subunits cause human hereditary hyperekplexia, a disorder characterized by an exaggerated startle response, which is a consequence of the role of GlyRs in motor reflex circuits of the spinal cord6.

Alternative names: Glycine Receptor alpha1, Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1, Glyr

Gene ID: 25674

NCBI Accession: NM_000171

UniProt: P07727

Pathways: Synaptic Membrane