HCN4 antibody (Intracellular)

Catalog No. ABIN7043283

Product Details anti-HCN4 Antibody

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Binding Specificity: AA 119-155, Intracellular

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This HCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Guinea Pig Polyclonal Antibody to HCN4 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: mouse - 33,Rabbit - identical, rat - 35,37 amino acid residues identical

Characteristics: Guinea pig Anti-HCN4 Antibody , raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN4 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-HCN4 Antibody (ABIN7043284, ABIN7044963 and ABIN7044964) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence HGHLHDSAEE RRLIAEGDASPGEDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235798)

Blocking Peptide: (ABIN7235798)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4 (HCN4 Products)

Background: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4,Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share a homology of about 60 % . However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .4,5mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.6

Alternative names: HCN4, Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Gene ID: 59266

NCBI Accession: NM_005477

UniProt: Q9Y3Q4