ITPR2 antibody (Intracellular)

Catalog No. ABIN7043288

Product Details anti-ITPR2 Antibody

Target: ITPR2 (Inositol 1,4,5-Trisphosphate Receptor, Type 2 (ITPR2))

Binding Specificity: AA 2683-2696, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITPR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to IP3 Receptor-2

Specificity: Intracellular, C-terminus

Predicted Reactivity: Mouse,human - 13,14 amino acid residues identical

Characteristics: Anti-IP3 Receptor-2 (ITPR2) Antibody (ABIN7043288, ABIN7044063 and ABIN7044064)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IP3R2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-CC116

Blocking Peptide: BLP-CC116

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ITPR2

Target: ITPR2 (Inositol 1,4,5-Trisphosphate Receptor, Type 2 (ITPR2))

Alternative Name: ITPR2 (ITPR2 Products)

Background: Inositol 1,4,5-trisphosphate receptor type 2, IP3R2, InsP3R2, IP3 receptor isoform 2,Inositol 1,4,5-trisphosphate receptors (IP3R) are intracellular Ca2+ release channels located on the endoplasmic reticulum (ER) and mediate Ca2+ mobilization from the ER to the cytoplasm in response to the binding of the second messenger, inositol 1,4,5-trisphosphate (IP3)1. IP3-induced Ca2+ release is triggered by various external stimuli, and most non-excitable cells use this mechanism as the primary Ca2+ signaling pathway. IP3Rs are therefore thought to have important physiological roles in various cell types and tissues2.Three subtypes of IP3Rs, derived from three distinct genes, have been identified in mammals3. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus are cytoplasmic. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required to maintain the suppression of channel activity4. Type 2 IP3R (IP3R2) is expressed in various tissues and cell lines. IP3R2 mRNA is localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary5. IP3R2 is active during osteoclast differentiation, and its absence causes a partial defect in osteoclastic differentiation.

Alternative names: IP3 Receptor-2 (ITPR2), Inositol 1,4,5-trisphosphate receptor type 2, IP3R2, InsP3R2, IP3 receptor isoform 2

Gene ID: 81678

NCBI Accession: NM_002223

UniProt: P29995

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma