NTSR1 antibody (Extracellular)

Catalog No. ABIN7043383

Product Details anti-NTSR1 Antibody

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Binding Specificity: AA 209-224, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NTSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Neurotensin Receptor 1

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Rat

Predicted Reactivity: rat - 12,16 amino acid residues identical,Mouse - 14

Characteristics: Anti-Neurotensin Receptor 1 (extracellular) Antibody (ABIN7043383, ABIN7044767 and ABIN7044768)) is a highly specific antibody directed against an epitope of the human NTSR1. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and flow cytometry applications. It has been designed to recognize NTSR1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT015

Blocking Peptide: BLP-NT015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NTSR1

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Alternative Name: NTSR1 (NTSR1 Products)

Background: NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR,Neurotensin receptor 1 (NTR1) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.NTR1 and NTR2 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1NTR1 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization, although coupling with Gi/o and to Gs has been observed in some expression systems.NTR1 is mainly expressed in the brain and gastrointestinal tract. In the brain, NTR1 mediates body temperature regulation, feeding behavior and locomotion regulation. One of the best studied roles of NTR1 is the regulation of dopamine release suggesting that NTR1 can be a suitable target for the development of a new class of anti-psychotics.2 In the periphery, NTR1 expression has been linked to the control of intestine motility and in the stimulation of growth of the intestine mucosa in both physiological and pathological conditions. NTR1 has been shown to be upregulated in several human cancers where it stimulates tumor growth in response to locally produced neurotensin peptide.3

Alternative names: Neurotensin Receptor 1, NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR

Gene ID: 4923

NCBI Accession: NM_002531

UniProt: P30989